Antiapoptotic effects of scutellarin on ultraviolet A-irradiated HaCaT human keratinocytes

• DOI: 10.1186/s41702-018-0022-y
• Publication Date: 2018-04-16T12:04:03Z
• Authors: Mina Choi; Seeun Jeon
• Keywords: HaCaT; Scutellarin; Digitonin; Viability assay; Comet Assay; Photoaging; Phototoxicity

This study examines the cellular protective effects of scutellarin on HaCaT cells in which oxidative stress was induced by ultraviolet A (UVA). Cell viability and lipid peroxidation were measured using WST-1 MDA assays, respectively. The changes cell number sub-G1 phase assessed cycle analysis, tailed DNA levels comet assay to examine degree damage. difference mitochondrial membrane potential examined JC-1 staining, regulation BAX mRNA expression quantitative real-time polymerase chain reaction. Although UVA-irradiated resulted a 3.63-fold increase peroxide, scutellarin-treated showed concentration-dependent decreases 2.06, 1.39, 0.97 times at concentrations 1, 5, 10 μM, increased 227% when irradiated UVA but decreased 187, 139, 120% treated with Tailed also effect. up 36% recovered 58, 82, 92% gene 9.7-fold downregulated 7.4-, 4.71-, 2.49-fold revealed cells. Further studies are warranted determine use as cosmeceutical ingredient.