Production of exopolysaccharides from novel marine bacteria and anticancer activity against hepatocellular carcinoma cells (HepG2)

• DOI: 10.1186/s42269-018-0032-3
• Publication Date: 2018-12-04T08:34:19Z
• Authors: Mohsen S. Asker; Osama H. El Sayed; Manal G. Mahmoud; Shaymaa M. Yahya; Sahar S. Mohamed; Manal S. Selim; Mohamed S. El Awady; Salma M. Abdelnasser; Mostafa M. Abo El...
• Keywords: Bacillus amyloliquefaciens; Pseudomonas fluorescens; HeLa

The aim of the current study based on production and characterization exopolysaccharides (EPSs) isolated from marine sediment Mediterranean Red Seas is to its cytotoxic activity against HepG2 cells. Eleven isolates have ability produce EPSs also decreased viability cell line in different manners. five most promising that high yield cytotoxicity were identified by 16S RNA as Brevundimonas subvibrioides MSA1, Bacillus thuringiensis E4, amyloliquefaciens MGA2, Pseudomonas fluorescens SGA3, Advenella Kashmirensis NRC-7. chemical composition following (M1, M3, M6, M15, M19, E2, E10, S5, S7, S11) demonstrates they are acidic sulfated heteropolysaccharides with relative ratios monosugars glucose, mannose, galactose, glucouronic acid, mannouronic acid. average molecular weights 1.94 × 104 7.95 105 g/mol number weight 1.51 7.53 g/mol. FTIR spectrum indicated presence sulfate carboxylic groups percentages. produced bacteria very for treating