Metabolomics approach to serum biomarker for laxative effects of red Liriope platyphylla in loperamide-induced constipation of SD rats

• DOI: 10.1186/s42826-019-0009-x
• Publication Date: 2019-08-07T10:02:39Z
• Authors: Ji Eun Kim; Young Ju Lee; Sung Ha Ryu; Ji Won Park; Mi Ju Kang; Hyeon Jun Choi; Su Ji Bae; Yusang Choi; Hyun Gu Kang; Kyu-Bong Kim; Suhkmann Kim; Yong Lim; Dae Youn Hwang
• Keywords: Loperamide; Laxative; Creatine

Red Liriope platyphylla (RLP) is a known herbal medicine used in the treatment of some chronic diseases including constipation, neurodegenerative disorders, diabetes and obesity. To determine characterize putative biomarkers that predict laxative effects induced by RLP treatment, alteration endogenous metabolites was measured serum loperamide (Lop)-induced constipation rats after administration extract (EtRLP) using 1H nuclear magnetic resonance (1H NMR) spectral data. The urine volume amounts, weights water contents stools were significantly recovered Lop + EtRLP treated group as compared to No group, whereas body weight food intake maintained constant levels. Also, significant recoveries thickness mucosa muscle detected colon group. Furthermore, pattern recognition showed absolutely different clustering analysis parameters when comparing Of 33 metabolites, 7 amino acids (alanine, arginine, glutamate, glutamine, glycine, threonine valine) 8 (betaine, creatine, glucose, taurine, ethanol, lactate, glycerol succinate) dramatically increased SD rats. These results provide first evidence pertaining metabolic changes with EtRLP. Additionally, these findings correlate observed 15 during