The secretome of induced pluripotent stem cells reduces lung fibrosis in part by hepatocyte growth factor

• DOI: 10.1186/scrt513
• Publication Date: 2014-11-10T17:10:43Z
• Authors: Amiq Gazdhar; Iwona Grad; Luca Tamò; Mathias Gugger; Anis Feki; Thomas Geiser
• Keywords: Male; 0301 basic medicine; Hepatocyte Growth Factor; Research; Induced Pluripotent Stem Cells; 610 Medicine & health; Fibrosis; Exocytosis; Rats, Inbred F344; Cell Line; Rats; 3. Good health; 03 medical and health sciences; Re-Epithelialization; Animals; Humans; Lung

Idiopathic pulmonary fibrosis (IPF) is a progressive and irreversible fibrotic lung disease, resulting in respiratory insufficiency reduced survival. Pulmonary result of repeated alveolar epithelial microinjuries, followed by abnormal regeneration repair processes the lung. Recently, stem cells their secretome have been investigated as novel therapeutic approach fibrosis. We evaluated potential induced pluripotent (iPSC) conditioned media (iPSC-cm) to regenerate epithelium vitro improve bleomycin injury vivo.IPSC-cm was collected from cultured iPSC derived human foreskin fibroblasts its biological effects on wound studied an healing assay vitro. Furthermore, iPSC-cm intratracheally instilled 7 days after rat lungs histologically biochemically assessed instillation.iPSC-cm increased compared with medium control. Intratracheal instillation bleomycin-injured collagen content improved vivo. Profibrotic TGFbeta1 α-smooth muscle actin (α-sma) expression were markedly treated group Antifibrotic hepatocyte growth factor (HGF) detected biologically relevant levels, specific inhibition HGF attenuated antifibrotic effect iPSC-cm, indicating central role iPSC-cm.iPSC-cm vivo, partially due presence may represent promising novel, cell free option against