Enrichment for a CD26hi SIRP− subset in lymph dendritic cells from the upper aero-digestive tract

• DOI: 10.1189/jlb.0404223
• Publication Date: 2004-06-15T18:26:13Z
• Authors: Mathieu Epardaud; Michel Bonneau; Fabrice Payot; Corinne Cordier; Jérôme Mégret; Chris Howard; Isabelle Schwartz...
• Keywords: Cholera toxin

Dendritic cells (DC) have been reported to migrate in afferent lymph the steady state. However, it is unknown whether DC traffic modulated by nature of drained tissue. To analyze influence mucosal versus cutaneous microenvironments on constitutive release, we exploited a novel technique cannulation sheep, which allowed comparison migrating from head mucosae [cervical (CerDC)] with skin [prescapular (PresDC)]. The migration rate was lower for CerDC than PresDC. Compared PresDC, contained higher proportion CD26hi signal regulatory protein (SIRP)- subset. It interesting that cytoplasmic apoptotic DNA as well cytokeratin-positive inclusions were primarily detected among SIRP- DC, an observation similar made rats, leads suggestion this subset involved self-antigen presentation and tolerance induction. After inoculation cholera toxin (CT) onto oro-nasal mucosae, CD26lo SIRP+ accelerated lymph, indicating effect CT mobilization not subset-specific. Our results show environment influences output relative representation lymph. This modulation nodes surfaces likely affect bias immune responses.