Stromal cell-derived factor-1/CXCL12 directly enhances survival/antiapoptosis of myeloid progenitor cells through CXCR4 and Gαi proteins and enhances engraftment of competitive, repopulating stem cells

• DOI: 10.1189/jlb.1002495
• Publication Date: 2003-04-24T19:20:31Z
• Authors: Hal E Broxmeyer; Lisa Kohli; Chang H Kim; Younghee Lee; Charlie Mantel; Scott Cooper; Giao Hangoc; Montaser Shaheen; Xiaxin Li; D Wade Clapp
• Keywords: Ex vivo; Interleukin 3

Stromal cell-derived factor-1 (SDF-1/CXCL12) enhances survival of myeloid progenitor cells. The two main questions addressed by us were whether these effects on the progenitors direct-acting and if SDF-1/CXCL12 enhanced engrafting capability competitive, repopulating mouse stem cells subjected to short-term ex vivo culture with other growth factors. had survival-enhancing/antiapoptosis human bone marrow (BM) cord blood (CB) BM colony-forming units (CFU)-granulocyte macrophage, burst-forming units-erythroid, CFU-granulocyte-erythroid-macrophage-megakaryocyte similar dose responses. direct-acting, as assessed colony formation single isolated CB CD34(+++) Effects mediated through CXCR4 G(alpha)i proteins. Moreover, greatly long-term, marrow-competitive, cultured interleukin-6 steel factor for 48 h. These results extend information SDF-1/CXCL12-CXCR4 axis may be relevance expansion gene-transduction procedures.