Long-term in vitro and in vivo effects of γ-irradiated BCG on innate and adaptive immunity

• DOI: 10.1189/jlb.4ma0215-059r
• Publication Date: 2015-06-17T03:25:22Z
• Authors: Rob J W Arts; Bastiaan A Blok; Peter Aaby; Leo A B Joosten; Dirk de Jong; Jos W M van der Meer; Christine Stabell...; Reinout van Crevel; Mihai G Netea
• Keywords: Heterologous

Abstract BCG vaccination is associated with a reduced mortality from nonmycobacterial infections. This likely to be mediated by combination of innate-immune memory (“trained immunity”) and heterologous effects on adaptive immunity. As such, could used boost host immunity but not in immunocompromised hosts, as it live, attenuated vaccine. Therefore, we assessed whether killed γBCG has similar potentiating effects. In an vitro model trained immunity, human monocytes were incubated for 24 h restimulated after 6 d. Cytokine production the role pattern recognition receptors histone methylation markers assessed. The vivo studied proof-of-principle trial 15 healthy volunteers. induced via NOD2 receptor pathway up-regulation H3K4me3 methylation. However, these less strong than those live BCG. volunteers had only minimal innate whereas significant increase Th1/Th17 was observed. Our results indicate that induces long-term training vitro. vivo, mainly adaptive-immune system, cytokine are limited.