sDR5-Fc inhibits macrophage M1 polarization by blocking the glycolysis.

• DOI: 10.11909/j.issn.1671-5411.2021.04.003
• Publication Date: 2021-04-28
• Authors: Guangyao Zhai; Shuyan Qie; Qianyun Guo; Yue Qi; Yujie Zhou
• Keywords: Macrophage polarization

BACKGROUND M1 polarization of macrophages is an important pathological process in myocardial ischemia reperfusion injury, which the major obstacle for treatment acute infarction. Currently, strategies and mechanisms inhibiting M1 are poorly explored. This study aims to investigate role soluble death receptor 5-Fc (sDR5-Fc) regulating under extreme conditions explore from aspect glycolysis. METHODS Extreme were induced RAW264.7 cells. Real-time quantitative polymerase chain reaction western blot used detect expression mRNA proteins, respectively. Cell counting kit-8 was proliferation activity Expression levels inflammatory cytokines determined by enzyme-linked immunosorbent assay. RESULTS We found that sDR5-Fc rescues conditions, including nutrition deficiency, excessive peroxide, ultraviolet irradiation. In addition, administration inhibits lipopolysaccharide (LPS) interferon-gamma (IFN-γ), as markers CD86, CXC motif chemokine ligand 10, matrix metalloproteinase 9, tumor necrosis factor-α, well secretion factors interleukin (IL)-1β IL-6, significantly decreased. By further investigation mechanisms, results showed can recover LPS IFN-γ pH reduction, lactic acid elevation, increased hexokinase 2 glucose transporter 1, glycolysis macrophages. CONCLUSIONS sDR5-Fc blocking glycolysis, provides a new direction development injury.