Lipidomics reveals that adiposomes store ether lipids and mediate phospholipid traffic,

• DOI: 10.1194/jlr.m600413-jlr200
• Publication Date: 2007-01-09T01:54:40Z
• Authors: René Bartz; Wen-Hong Li; Barney Venables; John K. Zehmer; Mary R. Roth; Ruth Welti; Richard G.W. Ande...; Pingsheng Liu; Kent D. Chapman
• Keywords: Lipidome

Lipid droplets are accumulations of neutral lipids surrounded by a monolayer phospholipids and associated proteins. Recent proteomic analysis isolated suggests that they part dynamic organelle system is involved in membrane traffic as well packaging distributing the cell. To gain better insight into function droplets, we used combination mass spectrometry NMR spectroscopy to characterize lipid composition this compartment. In addition cholesteryl esters triacylglycerols with mixed fatty acid composition, found ∼10–20% were ether monoalk(en)yl diacylglycerol. Although contain only 1–2% weight, >160 molecular species identified quantified. Phosphatidylcholine (PC) was most abundant class, followed phosphatidylethanolamine (PE), phosphatidylinositol, ether-linked phosphatidylcholine (ePC). Relative total membrane, droplet enriched lysoPE, lysoPC, PC but deficient sphingomyelin, phosphatidylserine, phosphatidic acid. These results suggest play central role metabolism intracellular traffic.