Fatty acid transport protein 4 is dispensable for intestinal lipid absorption in mice

• DOI: 10.1194/jlr.m800400-jlr200
• Publication Date: 2008-10-09T01:39:27Z
• Authors: Jien Shim; Casey L. Moulson; Elizabeth P. Newb...; Meei-Hua Lin; Yan Xie; Susan M. Kennedy; Jeffrey H. Miner; Nicholas O. Davidson
• Keywords: Male; 0301 basic medicine; Mice, Transgenic; QD415-436; Lipoproteins, VLDL; Biochemistry; Mice; 03 medical and health sciences; fat; Animals; RNA, Messenger; Intestinal Mucosa; Triglycerides; Mice, Knockout; cholesterol; Fatty Acid Transport Proteins; Lipid Metabolism; Dietary Fats; Mice, Inbred C57BL; Kinetics; Cholesterol; Intestinal Absorption; Liver; transporter; Mice, Inbred CBA; dietary; Female

FA transport protein 4 (FATP4), one member of a multigene family transporters, was proposed as major transporter in intestinal lipid absorption. Due to the fact that Fatp4(-/-) mice die because perinatal skin defect, we rescued phenotype using an FATP4 transgene driven by keratinocyte-specific promoter (Fatp4(-/-);Ivl-Fatp4(tg/+) mice) elucidate role dietary Fatp4(-/-);Ivl-Fatp4(tg/+) and wild-type littermates displayed indistinguishable food consumption, growth, weight gain on either low or high fat (Western) diets, with no differences triglyceride (TG) absorption fecal losses. Cholesterol TG kinetics were between genotypes, although Western diet fed showed significant increase enterocyte content. There compensatory upregulation other FATP members any cholesterol transporters mice. Furthermore, serum levels lower mice, there difference hepatic VLDL secretion in-vivo content chow diet. Taken together, our studies find evidence for physiological