HDL is the major lipoprotein carrier of plasma F2-isoprostanes

• DOI: 10.1194/jlr.m800607-jlr200
• Publication Date: 2008-12-03T03:43:50Z
• Authors: Julie M. Proudfoot; Anne E. Barden; Wai Mun Loke; Kevin D. Croft; Ian B. Puddey; Trevor A. Mori
• Keywords: Isoprostanes; Paraoxonase; Isoprostanes

Enhanced oxidative stress is implicated in the development of atherosclerosis humans and animal models. F(2)-isoprostanes are formed vivo via free radical peroxidation arachidonic acid, their quantification has allowed assessment vivo. associate with lipids, although distribution human plasma lipoproteins unknown. Our aim was to determine levels isolated from by ultracentrifugation fast protein liquid chromatography (FPLC). were significantly higher HDL compared LDL or VLDL after isolation FPLC. Furthermore, HDL3 particles contained elevated HDL2. Platelet activating factor acetylhydrolase (PAF-AH), which hydrolyses esterified phospholipids, predominantly associated LDL. Reduced may be related PAF-AH activity Paraoxonase 1 (PON-1) HDL2 a contributing lower HDL3. Further studies required establish implications these findings on function.