Glycolaldehyde‐Modified Bovine Serum Albumin Downregulates Leptin Expression in Mouse Adipocytes via a CD36‐Mediated Pathway

• DOI: 10.1196/annals.1333.080
• Publication Date: 2005-07-21T16:59:10Z
• Authors: YUKA UNNO; MASAKAZU SAKAI; YU‐ICHIRO SAKAMOTO; AKIHIKO KUNIYASU; RYOJI NAGAI; HITOSHI NAKAYAMA; SEIKOH HORIUCHI
• Keywords: CD36; Scavenger Receptor; Bovine serum albumin; Troglitazone; Leptin receptor

A bstract : Previous observations by us have clarified that proteins modified advanced glycation end products (AGEs) are recognized as effective ligands CD36‐overexpressed CHO cells and undergo receptor‐mediated endocytosis. CD36, a member of the class B scavenger receptor family, also acts fatty acid transporter in adipocytes. Oxidized low‐density lipoprotein (Ox‐LDL), ligand for is known to upregulate CD36 activating peroxisome proliferator‐activated γ (PPAR‐γ) macrophages, whereas PPAR‐γ such troglitazone 15‐deoxy‐delta12,14‐prostaglandin J2 decrease leptin secretion from The purpose this study was examine effects AGE on expression Glycolaldehyde‐modified bovine serum albumin (GA‐BSA) decreased at both protein mRNA levels 3T3‐L1 adipocytes mouse epididymal binding subsequent endocytic degradation GA‐BSA were effectively inhibited neutralizing anti‐CD36 antibody. These results indicate interaction with leads downregulation adipocytes, suggesting AGE‐induced linked reduction insulin sensitivity metabolic syndrome.