Divergent Expression of Type 2 Deiodinase and the Putative Thyroxine-Binding Protein p29, in Rat Brain, Suggests that They Are Functionally Unrelated Proteins

• DOI: 10.1210/en.2002-220823
• Publication Date: 2003-02-13T22:05:31Z
• Authors: Ana Montero-Pedra...; Juan Bernal; Ana Guadaño-Ferraz
• Keywords: Selenoprotein

Deiodinases (D1, D2, and D3) are selenoproteins involved in thyroid hormone metabolism. Generation of the active T(3), from T(4), is carried out by D1 whereas D3 degrades both hormones. The identity cloned D2 as a selenoprotein well supported biochemical physiological data. However, an alternative view has proposed that type 2 deiodinase nonselenoprotein complex containing putative T(4) binding subunit called p29, with almost sequence Dickkopf protein Dkk3. To explore possible functional relationship between p29 we have compared their mRNA expression patterns rat brain. In brain, parenchyma was expressed neurons. High levels were found all regions blood-cerebrospinal fluid (CSF) barrier. present different types cells than exception tanycytes. Our data do not support D2. On other hand, blood-CSF barrier suggests it might be transport to CSF, but further studies needed substantiate this hypothesis.