In Vivo 17β-Estradiol Treatment Contributes to Podocyte Actin Stabilization in Female db/db Mice

• DOI: 10.1210/en.2012-1637
• Publication Date: 2012-10-16T06:03:17Z
• Authors: Paola Catanuto; Alessia Fornoni; Simone Pereira-Simon; Fayi Wu; Kerry L. Burnstein; Xiaomei Xia; Francesco Conti; Andrea Lenzi; Sharon Elliot
• Keywords: Glomerulosclerosis

Abstract We recently showed that 17β-estradiol (E2) treatment ameliorated type 2 diabetic glomerulosclerosis in mice part by protecting podocyte structure and function. Progressive damage is characterized foot process effacement, vacuolization, detachment of podocytes from the glomerular basement membrane, apoptosis. In addition, are highly dependent on preservation their actin cytoskeleton to ensure proper function survival. Because E2 administration prevented our study db/db has been shown regulate both apoptosis other cell types tissues, we investigated whether remodeling were isolated E2-treated mice. performed G-actin/F-actin assays, Western analysis for Hsp25 expression, Ras-related C3 botulinum toxin substrate 1 (Rac1) activity, assays previously vivo-treated placebo female found vivo protects against a phenotype change cultured percent increase F-actin vs. G-actin, suppression expression transcriptional activation, Rac1 decreased apoptotic intermediates. conclude these studies may prevent/reduce diabetes-induced kidney disease.