Differential gene expression of growth hormone (GH)-releasing hormone (GRH) and GRH receptor in various rat tissues.

• DOI: 10.1210/endo.136.9.7649123
• Publication Date: 2014-01-08T16:07:18Z
• Authors: S Matsubara; M Sato; M Mizobuchi; M Niimi; J Takahara
• Keywords: Northern blot

Growth hormone (GH)-releasing (GRH) acts on specific receptors in the anterior pituitary to stimulate synthesis and release of GH. Recent reports suggest that GRH is also synthesized extrahypothalamic tissues. To evaluate potential roles GRH, we studied gene expression various rat tissues by reverse transcribed (RT)-polymerase chain reaction (PCR). Total RNA was extracted from twenty-three organs RT-PCR performed with receptor primers. Highly-sensitive RT-PCR-Southern blotting showed mRNA coexist widespread (14 25 tissues). relatively abundant cerebral cortex, brain stem, testis, placenta, while renal medulla pelvis. Northern blot hybridization using poly A+ indicated transcript found similar longer (about 4 Kb) size. These results plays a role not only neuroendocrine axis, but autocrine paracrine systems