Regulation and Expression of Gonadotropin-Releasing Hormone Gene Differs in Brain and Gonads in Rainbow Trout*

• DOI: 10.1210/endo.140.7.6795
• Publication Date: 2014-01-08T16:10:08Z
• Authors: Kristian R. Von S...; Nancy M. Sherwood
• Keywords: Male; 0303 health sciences; DNA, Complementary; Base Sequence; Transcription, Genetic; Molecular Sequence Data; Ovary; Brain; Gene Expression; Gene Expression Regulation, Developmental; Gonadotropin-Releasing Hormone; 03 medical and health sciences; Isomerism; Oncorhynchus mykiss; Testis; Animals; Female; Amino Acid Sequence; Promoter Regions, Genetic

The GnRH gene is transcribed in both the brain and gonads. critical for reproduction, but function importance of ovary testis not clear. In this study we examine whether regulation distinct gonads, gonads altered after genome duplication, regulatory region tightly conserved vertebrates. From testis, isolated sequenced first time two different genes their complementary DNAs that encode identical peptide known as salmon GnRH. Rainbow trout were selected because they are tetraploid due to duplication. A downstream promoter used by messenger RNA1 (mRNA1) mRNA2, mRNA2 also uses an upstream only Two types long transcripts use alternative start site at position 323; one these retains intron 1. This 5'-untranslated a likely mRNA gonad. Additional evidence separate expression pattern exists mRNAs during development maturation. Gene duplication did alter encoded peptide, changed resulted complete divergence sequence from -215. comparison mammalian reveals promoters without identity except few consensus sites regions. duplicated provide model whose product controls reproduction all