Tudor and Nuclease-Like Domains Containing Protein p100 Function as Coactivators for Signal Transducer and Activator of Transcription 5

• DOI: 10.1210/me.2002-0256
• Publication Date: 2003-06-24T00:42:51Z
• Authors: Kirsi Paukku; Jie Yang; Olli Silvennoinen
• Keywords: STAT5; Transcription

Signal transducer and activator of transcription 5 (Stat5) plays a critical role in prolactin (PRL)-induced several milk protein genes. Stat5-mediated gene regulation is modulated by cooperation Stat5 with cell type- promoter-specific factors as well interaction transcriptional coregulators. Recently, the expression tudor staphylococcal nuclease-like domains containing p100 was found to be increased mammary epithelial cells during lactation response lactogenic hormones. initially identified coactivator Epstein-Barr virus nuclear antigen 2. In this study we investigated potential PRL-induced activation. PRL stimulation levels HC11 mouse cells. did not affect early activation events Stat5, but enhanced Stat5-dependent associated both vivo vitro, mediated p100. Together these results suggest that functions for existence positive regulatory loop transcription, which stabilizes protein, turn can cooperate