Dysregulation of EGF Family of Growth Factors and COX-2 in the Uterus during the Preattachment and Attachment Reactions of the Blastocyst with the Luminal Epithelium Correlates with Implantation Failure in LIF- Deficient Mice

• DOI: 10.1210/mend.14.8.0498
• Publication Date: 2014-01-08T17:30:44Z
• Authors: Haengseok Song; Hyunjung Lim; Sanjoy K. Das; Bibhash C. Paria; Sudhansu K. Dey
• Keywords: Epiregulin; Juxtacrine signalling; Decidualization; Decidual cells

Abstract Various mediators, including cytokines, growth factors, homeotic gene products, and prostaglandins (PGs), participate in the implantation process an autocrine, paracrine, or juxtacrine manner. However, interactions among these factors that result successful are not clearly understood. Leukemia inhibitory factor (LIF), a pleiotropic cytokine, was shown to be expressed uterine glands on day 4 morning before is critical this mice. mechanism by which LIF executes its effects remains unknown. Moreover, of with other implantation-specific molecules have yet been defined. Using normal delayed models, we herein show only progesterone (P4)-primed response nidatory estrogen, it also induced stromal cells surrounding active blastocyst at time attachment reaction. This suggests has biphasic effects: first preparation receptive uterus subsequently The participates events addressed using LIF-deficient We observed while cell-specific proliferation, steroid hormone responsiveness, expression patterns several genes normal, specific members EGF family such as amphiregulin (Ar), heparin-binding EGF-like (HB-EGF), epiregulin, LIF(−/−) uteri during anticipated implantation, although receptor (erbBs) correctly. Furthermore, cyclooxygenase-2 (COX-2), inducible rate-limiting enzyme for PG synthesis essential aberrantly These results suggest dysregulation COX-2 contributes, least partially, failure Since estrogen receptivity, induction, activation, possible P4-primed mediated via signaling. can partially resume P4-primed, implanting mice absence suggesting induction one many functions executed implantation.