Ancestry, Haplogroups and HLA-DRB1 Alleles of Multiple Sclerosis in Bogotá, Colombia. (P2.221)

• DOI: 10.1212/wnl.84.14_supplement.p2.221
• Publication Date: 2023-12-06T23:42:28Z
• Authors: Jaime Toro; Lisseth Burbano; Camilo Diaz-Cruz; Saúl Reyes; David Cuéllar-Gir...; Diana Narváez; Fabián Cortés; Helena Groot
• Keywords: Haplogroup; HLA-DRB1

OBJECTIVE: To determine ancestry informative markers, haplogroups and frequency of HLA-DRB1 alleles in patients with Multiple Sclerosis (MS) Bogotá, Colombia. BACKGROUND: In European-descended populations the HLA-DRB1*15 polymorphism has been identified as strongest genetic risk factor for MS, whereas HLA-DRB1*14 allele attenuates MS risk. However, strength association these have not reported from low prevalence highly admixed region DESIGN/METHODS: Genomic DNA was isolated purified blood samples by a standard salting out procedure. genotyping done using Single Specific Primer Polymerase Chain Reaction. Mitochondrial hypervariable region-1 amplified were determined HaploGrep software. Ancestry proportions estimated panel markers. test HLA polymorphisms separate multivariate logistic regression models fitted adjusting age at enrollment, gender, smoking history (ever vs never) ancestry. Bonferroni correction used to account multiple tests. RESULTS: 100 (mean 40.4±12 years; 70[percnt] females) 200 healthy controls 37.6±11 83.5[percnt] included analysis. present 31[percnt] cases 13,5[percnt] 5[percnt] 15,5[percnt] controls. Using significance threshold p<0.004, significantly associated multivariable model (OR=3.05, p<0.001) while confirmed protective (OR=0.16, p =0.001). CONCLUSIONS: We MS. addition, we report role allele. This latter finding could partially explain Study Supported by: Universidad El Bosque, de los Andes Fundación Santa Fe Bogotá.