The Effect of IL-10 on M2-Activated Human Microglia (S12.006)

• DOI: 10.1212/wnl.84.14_supplement.s12.006
• Publication Date: 2023-12-06T23:42:28Z
• Authors: Luke Healy; Craig Moore; Hanane Touil; Samuel Ludwin; Amit Bar-Or; Jack Antel

OBJECTIVE: To investigate the effect of IL-10 on phenotypic characteristics M2 polarized human microglia. BACKGROUND: Myeloid cells acquire distinct immunologic phenotypes dependent their state 'polarization': either 'M1' (pro-inflammatory) or 'M2' (anti-inflammatory). cells, generated using mCSF/IL-4/IL13, produce increased quantities anti-inflammatory cytokines and display phagocytic activity expression scavenger receptors. Previous in vitro studies show that mCSF/IL-4/IL13 treated microglia express more limited functional properties than blood-derived macrophages. Rodent-based have defined subsets (M2a, M2b M2c) within overall population thus far not been investigated system. Human M2c macrophages induced by treatment with combination (M2a protocol) enhancement aforementioned M2-like properties. DESIGN/METHODS: were cultured from both fetal adult brain tissue; these exposure to ± (50ng/ml). Phenotypic markers (cell surface intra-cellular) assessed way multi-colour flow cytometery qPCR. In situ myeloid was demonstrated immunohistochemical staining post-mortem CNS tissue. RESULTS: We able demonstrate higher specific markers, CD163 HMOX-1, presence compared M2a conditions alone; CD206 CD209 comparably expressed. Analysis a case Schistosoma mekongi parasitic infection confirmed is selectively expressed its elevated under pathologic condition promotes Th2 responses. CONCLUSIONS: Our findings induces vitro, pathological associated levels are also likely induce such situ.