[The low expression of long non-coding RNA Linc00638 contributes to rheumatoid arthritis progression by regulating inflammation and oxidative stress].

• DOI: 10.12122/j.issn.1673-4254.2021.07.01
• Publication Date: 2021-07-20
• Authors: Yanqiu Sun; J Liu; Ling Xin; Qiao Zhou; X Chen; Xiang Ding; X Zhang
• Keywords: Arthritis, Rheumatoid; Inflammation; Oxidative Stress; Leukocytes, Mononuclear; Humans; RNA, Long Noncoding; Middle Aged

To investigate the expression of long non-coding RNA (lncRNA) Linc00638 in rheumatoid arthritis (RA) and its regulatory role inflammation oxidative stress synovial fibroblasts RA patients (RA-FLS).Peripheral blood mononuclear cells (PBMCs) were collected from 20 healthy individuals 35 for detecting using RT-qPCR to analyze correlation with clinical indicators patients. A overexpression plasmid siRNA targeting transfected into RA-FLS, changes cell viability was observed CCK8 assay; levels interleukin-4 (IL-4), IL-6, reactive oxygen species (ROS) superoxide dismutase (SOD) supernatant detected ELISA.Compared control subjects, had significantly increased ESR, CRP, RF, anti-CCP, IgA, C4 (P < 0.05) decreased PBMCs 0.01). The area under receiver-operating characteristic (ROC) curve 91.86% best cut-off value 0.74 diagnosis RA. Spearman analysis showed that level negatively correlated age, course disease, DAS28, RF positively IL-4 SOD 0.05). Association rule a strongly an increase age (>60 years), longer disease (>10 elevated SOD. In inhibited while interference obviously enhanced viability. Over-expression also expressions IL-6 ROS 0.05), produced opposite effects 0.05).RA have low Linc00638, which may participate progression by regulating stress.