[Licochalcone A induces cell cycle arrest in human lung squamous carcinoma cells via the PI3K/Akt signaling pathway].

• DOI: 10.12122/j.issn.1673-4254.2023.01.15
• Publication Date: 2023-01-20
• Authors: Fan Xiao; Jiawei Wang; L Wang
• Keywords: MTT assay; Cyclin D; Viability assay; Cyclin E

To investigate the effect of licochalcone A (LCA) on proliferation and cell cycle human lung squamous carcinoma cells explore its possible molecular mechanism.MTT assay was used to detect changes in H226 after treatment with different concentrations LCA for 48 h, IC50 calculated. Flow cytometry analyze treated 10, 20, 40 μmol/L LCA, expressions cyclin D1, cyclin-dependent kinase CDK2 CDK4, p-PI3K, PI3K, p-Akt, Akt were detected using Western blotting. The intraperitoneal injection 24 days tumor volume weight assessed a BALB/c-nu mouse model bearing xenografts.MTT showed that significantly decreased viability an 28.3 at h. suggested induced obvious arrest G1 phase. also CDK2, inhibited phosphorylation PI3K cells. In tumor-bearing mice, reduced weight.LCA is capable inhibiting inducing possibility by regulating PI3K/Akt singling pathway.