The Effect of Ketamine Anesthesia on the Immune Function of Mice with Postoperative Septicemia

• DOI: 10.1213/ane.0b013e3181ed12fc
• Publication Date: 2010-08-13T09:49:24Z
• Authors: Tetsuya Takahashi; Manabu Kinoshita; Satoshi Shono; Yoshiko Habu; Takahiro Ogura; Shuhji Seki; Tomiei Kazama
• Keywords: Lipopolysaccharides; Male; Methyl Ethers; Laparotomy; 3. Good health; Mice, Inbred C57BL; Mice; Sevoflurane; 03 medical and health sciences; Postoperative Complications; 0302 clinical medicine; Sepsis; Escherichia coli; Animals; Cytokines; Anesthesia; Ketamine; Escherichia coli Infections

It is unknown how ketamine anesthesia immunologically affects the outcome of patients with postoperative septicemia. We investigated effects on mice an Escherichia coli or lipopolysaccharide (LPS) challenge after laparotomy, focusing phagocytosis by liver macrophages (Kupffer cells) and cytokine production.C57BL/6 received sevoflurane during which was followed E. LPS challenge; thereafter, mouse survival rates secretions were examined. The a β-adrenoceptor antagonist, nadolol, also assessed to clarify mechanisms ketamine-induced immunosuppressive effects.Ketamine increased rate laparotomy compared anesthesia, whereas such effect not observed challenge. Ketamine suppressed tumor necrosis factor (TNF) interferon (IFN)-γ secretion When bacterial growth inhibited using antibiotic, effectively improved anesthesia. Neutralization TNF decreased IFN-γ in antibiotic-treated suggesting that ketamine's suppression may improve survival. vivo microspheres Kupffer cells LPS-challenged mice. Concomitant use nadolol anesthetic dose did restore mice, mechanism independent β-adrenergic pathway. However, it restored under low-dose (10% dose). In contrast, decrease cells, induced via pathway, distinct mechanisms.Ketamine suppresses production cells/macrophages. Therefore, unless well controlled (by antibiotic), infection might despite reduction inflammatory response.