Sequential events during the quiescence to proliferation transition establish patterns of follicle cell differentiation in the Drosophila ovary

• DOI: 10.1242/bio.059625
• Publication Date: 2022-12-16T11:30:42Z
• Authors: Eric H. Lee; Daniel Zinshteyn; Fred Miglo; Melissa Q. Wang; Jessica Reinach; Cindy M. Chau; Joseph M. Grosste...; Iliana Correa; Kelly Costa; Alberto Vargas; Aminah Johnson; Sheila M. Longo; Jennifer I. Alexa...; Alana M. O'Reilly
• Keywords: 0301 basic medicine; cell fate; follicle formation; oogenesis; QH301-705.5; cytoplasmic projections; Science; Q; Ovary; Cell Differentiation; self-renewal; stem cell; quiescence to proliferation transition; 03 medical and health sciences; Drosophila melanogaster; Animals; Drosophila Proteins; Female; Drosophila; Biology (General); Cell Division; Research Article

Stem cells cycle between periods of quiescence and proliferation to promote tissue health. In Drosophila ovaries, transitions follicle stem (FSCs) are exquisitely feeding-dependent. Here, we demonstrate feeding-dependent induction cell differentiation markers, eyes absent (Eya) castor (Cas) in FSCs, a patterning process that does not depend on induction. Instead, FSCs extend micron-scale cytoplasmic projections dictate Eya-Cas patterning. We identify still life sickie as necessary sufficient for FSC projection growth Our results suggest sequential, interdependent events establish long-term patterns precursors, independently