Cdc42/N-WASP signaling links actin dynamics to pancreatic β cell delamination and differentiation

• DOI: 10.1242/dev.100297
• Publication Date: 2014-01-21T12:23:08Z
• Authors: Gokul Kesavan; Oliver Lieven; Anant Mamidi; Zarah Löf Öhlin; Jenny Kristina Jo...; Wan-Chun Li; Silvia Lommel; Thomas Uwe Greiner; Henrik Semb
• Keywords: CDC42

Delamination plays a pivotal role during normal development and cancer. Previous work has demonstrated that delamination epithelial cell movement within the plane of an epithelium are associated with change in cellular phenotype. However, how this positional is linked to differentiation remains unknown. Using developing mouse pancreas as model system, we show β two independent events, which controlled by Cdc42/N-WASP signaling. Specifically, expression constitutively active Cdc42 cells inhibits differentiation. These processes normally junctional actin cell-cell junction disassembly fate-determining transcription factors, such Isl1 MafA. Mechanistically, demonstrate genetic ablation N-WASP expressing partially restores both findings elucidate dynamics via signaling cell-autonomously control not only but also mammalian organogenesis.