A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus

• DOI: 10.1242/dev.105.1.61
• Publication Date: 2021-04-23T18:23:36Z
• Authors: Joseph A. Dent; Andrew G. Polson; Michael W. Klymko...
• Keywords: Intermediate Filament Protein; Mesenchyme

Abstract We have developed a whole-mount immunocytochemical method for Xenopus and used it to map the expression of intermediate filament protein vimentin during early embryogenesis. two monoclonal antibodies, 14h7 RV202. Both label filaments in A6 cells. RV202 reacts specifically with (Mr, 55×103) on Western blots cells embryos. second, insoluble polypeptide 57×103Afr found The 57 ×103Afr appears be an immunochemically related vimentin. In embryo, we first at time neural tube closure (stage 19) located lateral margins tube. By stage 26, these cells, which are presumably radial glia, present along entire length tail bud. Cells optic vesicles express by 24. Vimentin-expressing mesenchymal appear surface somites 22/23; anterior progressively more posterior as development continues. Beginning 24, ventral associated pronephric ducts; below later somites. dorsal fin mesenchyme expresses 26. head, both mesodermally-derived neural-crest-derived tissues These include branchial arches, mandibular arch, corneal epithelium, eye, meninges surrounding otic vesicle. 33, vimentin-expressing pericardial cavity line vitelline veins. Vimentin marker differentiation subset central nervous system head body embryo.