HGF/SF: a potent cytokine for mammary growth, morphogenesis and development

• DOI: 10.1242/dev.121.9.2897
• Publication Date: 2021-04-26T02:25:19Z
• Authors: Birunthi Niranjan; Lakjaya Buluwela; Jeffrey Yant; Nina Perusinghe; Amanda Atherton; Deborah Phippard; Trevor Dale; Barry Gusterson; Tahereh Kamalati
• Keywords: 0303 health sciences; Base Sequence; Hepatocyte Growth Factor; Molecular Sequence Data; Gene Expression; Fibroblasts; Proto-Oncogene Proteins c-met; Blotting, Northern; Polymerase Chain Reaction; Epithelium; Cell Line; 03 medical and health sciences; Dogs; Mammary Glands, Animal; Cell Movement; Morphogenesis; Animals; Humans; Female; Breast; Cell Division; DNA Primers

ABSTRACT The mammary gland is a renewing tissue in which morphogenetic processes and differentiation occur cyclically during the menstrual cycle, pregnancy lactation. These events have been shown to be dependent upon epithelial-mesenchymal interactions. Studies of effects individual factors, their cellular source target cell populations different developmental stages are greatly facilitated by accessibility this organ application new techniques that allow purification major epithelial stromal components tissue. Here we demonstrate HGF/SF its receptor, c-met, expressed regulated temporally mouse development differentiation. We show human fibroblasts produce not only mitogenic but morphogenic motogenic for both cells. found luminal myoepithelial cells express c-met differentially has on these two populations. cells, This discussed context proliferative compartments normal potential role act as skeleton ductal development.