Role for mRNA localization in translational activation but not spatial restriction of nanos RNA

• DOI: 10.1242/dev.126.4.659
• Publication Date: 2021-04-26T03:59:51Z
• Authors: Sherri Evans Berg...; Elizabeth R. Gavis
• Keywords: Germ plasm; Drosophila embryogenesis

Abstract Patterning of the anterior-posterior body axis during Drosophila development depends on restriction Nanos protein to posterior early embryo. Synthesis occurs only when maternally provided nanos RNA is localized pole by a large, cis-acting signal in 3′ untranslated region (3′UTR); translation unlocalized repressed 90 nucleotide Translational Control Element (TCE), also 3′UTR. We now show quantitatively that majority embryo not but distributed throughout cytoplasm, indicating translational repression primary mechanism for restricting production posterior. Through an analysis transgenes bearing multiple copies 3′UTR regulatory sequences, we provide evidence localization components posteriorly germ plasm activates its preventing interaction with repressors. This mutually exclusive relationship between and mediated 180 signal, containing TCE. These studies suggest ability direct wild-type patterning requires recognition multiple, unique elements within novel factors. Finally, propose differences efficiencies which different RNAs are result from use temporally distinct pathways oogenesis.