Perturbing phosphoinositide homeostasis oppositely affects vascular differentiation in Arabidopsis thaliana roots

• DOI: 10.1242/dev.155788
• Publication Date: 2017-08-30T00:20:10Z
• Authors: Bojan Gujas; Tiago M. D. Cruz; Elizabeth Kastanaki; Joop E. M. Vermeer; Teun Munnik; Antia Rodriguez-V...
• Keywords: Vascular tissue; Homeostasis

The plant vascular network consists of specialized phloem and xylem elements that undergo two distinct morphogenetic developmental programs to become transport-functional units. Whereas vacuolar rupture is a determinant step in protoxylem differentiation, protophloem never form big central vacuole. Here, we show genetic disturbance phosphatidylinositol 4,5-bis-phosphate [PtdIns(4,5)P2] homeostasis rewires cell trafficking towards the vacuole Arabidopsis thaliana roots. Consequently, an enhanced phosphoinositide-mediated biogenesis correlates with premature programmed death (PCD) secondary wall elaboration cells. By contrast, fusion events cells trigger abnormal formation vacuoles, preventing clearance tissue functionality. Removal inositol 5' phosphatase COTYLEDON VASCULAR PATTERN 2 from plasma membrane (PM) by brefeldin A (BFA) treatment increases PtdIns(4,5)P2 content at PM disrupts continuity. Conversely, BFA application abolishes without PCD, suggesting existence additional PtdIns(4,5)P2-dependent mechanisms. Overall, our data indicate tight phosphoinositide required modulate intracellular contributing oppositely regulate differentiation.