The formin DAAM is required for coordination of the actin and microtubule cytoskeleton in axonal growth cones

• DOI: 10.1242/dev.157982
• Publication Date: 2017-08-15T11:25:13Z
• Authors: Szilárd Szikora; István Földi; Krisztina Tóth; Ede Migh; Andrea Vig; Beáta Bugyi; József Maléth; Péter Hegyi; Péter Kaltenecker; Natalia Sanchez-S...; József Mihály
• Keywords: Formins; Growth cone

Directed axonal growth depends on correct coordination of the actin and microtubule cytoskeleton in cone.However, despite relatively large number proteins implicated actin-microtubule crosstalk, mechanisms whereby polymerization is coupled to stabilization advancement peripheral cone remained largely unclear.Here, we identified formin Dishevelled-associated activator morphogenesis (DAAM) as a novel factor playing role concerted regulation remodeling Drosophila melanogaster primary neurons.In vitro, DAAM binds F-actin well microtubules has ability crosslink two filament systems.Accordingly, associates with neuronal cytoskeleton, significant fraction accumulates at places where filaments overlap that microtubules.Loss affects morphology, several aspects dynamics; biochemical cellular assays revealed activity binding tip protein EB1.Together, these data suggest that, besides operating an assembly factor, involved linking filopodia during growth.