The Shh receptor Boc is important for myelin formation and repair

• DOI: 10.1242/dev.172502
• Publication Date: 2019-05-02T14:29:14Z
• Authors: Mary Zakaria; Julien Ferent; Ines Hristovska; Yousra Laouarem; Amina Zahaf; Abdelmoumen Kasso...; Marie-Eve Mayeur; Olivier Pascual; Frederic Charron; Elisabeth Traiffort
• Keywords: Male; Mice, Knockout; 0301 basic medicine; Macrophages; Blotting, Western; Intermediate Filaments; Cell Differentiation; Receptors, Cell Surface; Mice, Inbred C57BL; Cuprizone; Mice; Oligodendroglia; 03 medical and health sciences; Immunoglobulin G; Animals; Hedgehog Proteins; Microglia; Cells, Cultured; Myelin Sheath; Demyelinating Diseases

ABSTRACT Myelination leads to the formation of myelin sheaths surrounding neuronal axons and is crucial for function, plasticity repair central nervous system (CNS). It relies on interaction oligodendrocytes: glial cells producing CNS myelin. Here, we have investigated role a component Sonic hedgehog (Shh) signalling pathway, co-receptor Boc, in developmental repairing myelination. During development, Boc mutant mice display transient decrease oligodendroglial cell density together with delayed Despite recovery at later stages, adult mutants still exhibit lower production basic protein correlated significant calibre callosal reduced amount neurofilament NF-M. repair, altered OPC differentiation observed reminiscent phenotype after blockade Shh signalling. In addition, microglia/macrophages unexpectedly apparent inability transition from highly faintly ramified morphology vivo. Altogether, these results identify as an important repair.