MicroRNAs miR-25, let-7 and miR-124 regulate the neurogenic potential of Müller glia in mice

• DOI: 10.1242/dev.179556
• Publication Date: 2019-08-05T17:35:10Z
• Authors: Stefanie G. Wohl; Marcus J. Hooper; Thomas A. Reh
• Keywords: Muller glia

Müller glial cells (MG) generate retinal progenitor (RPC)-like after injury in non-mammalian species, though this does not occur the mammalian retina. Studies have profiled gene expression these to define genes that may be relevant their differences neurogenic potential. However, less is known about micro-RNA (miRNA) expression. In study, we compared miRNAs from RPCs and MG identify more highly expressed RPCs, others MG. To determine whether are difference potential between two cell types, tested them dissociated cultures of using either mimics or antagomiRs increase reduce expression, respectively. Among tested, miR-25 miR-124 over-expression, let-7 antagonism, induced Ascl1 conversion approximately 40% mature into a neuronal/RPC phenotype. Our results suggest miRNA contribute manipulations provide new tool reprogram for regeneration.