The simultaneous interaction of MSL2 with CLAMP and DNA provides redundancy in the initiation of dosage compensation in Drosophila males

• DOI: 10.1242/dev.179663r1
• Publication Date: 2025-01-21T08:25:24Z
• Authors: Evgeniya Tikhonova; Anna Fedotova; Artem Bonchuk; Vladic Mogila; Erica N. Larschan; Pavel Georgiev; Oksana Maksimenko
• Keywords: Dosage compensation; Clamp

ABSTRACT The binding of the Drosophila male-specific lethal dosage compensation complex (DCC) exclusively to male X chromosome provides an excellent model system understand mechanisms selective recruitment protein complexes chromatin. Previous studies showed that organizer complex, MSL2, and ubiquitous DNA-binding CLAMP are key players in specificity binding. CXC domain MSL2 binds genomic sites DCC vitro. Another conserved named Clamp-binding (CBD) directly interacts with N-terminal zinc-finger CLAMP. Here, we found inactivation CBD or individually only modestly affected males. However, combination these two genetic lesions within same mutant resulted increased loss chromosome. Thus, proper positioning requires interaction either DNA initiate