c-Src controls stability of sprouting blood vessels in the developing retina independently of cell-cell adhesion through focal adhesion assembly

• DOI: 10.1242/dev.185405
• Publication Date: 2020-02-27T17:45:18Z
• Authors: Lilian Schimmel; Daisuke Fukuhara; Mark Richards; Yi Jin; Patricia Essebier; Emmanuelle Frampton; Marie Hedlund; Elisabetta Dejana; Lena Claesson-Welsh; Emma Gordon
• Keywords: Sprouting

Endothelial cell adhesion is implicated in blood vessel sprout formation, yet how controls angiogenesis, and whether it occurs via rapid remodeling of adherens junctions or focal assembly, both, remains poorly understood. Furthermore, endothelial controlled particular tissues under different conditions unexplored. Here, we have identified an unexpected role for spatiotemporal c-Src activity sprouting angiogenesis the retina, which contrast to dominant focus on maintenance vascular integrity. Thus, mice specifically deficient displayed significantly reduced loss actin-rich filopodial protrusions at front developing retina. In what has been observed during leakage, cell-cell was unaffected by c-Src. Instead, decreased angiogenic due assembly cell-matrix adhesion, resulting stability. These results demonstrate that signaling specified membrane compartments (adherens adhesions) control processes a tissue- context-dependent manner.