Human macrophages rescue myoblasts and myotubes from apoptosis through a set of adhesion molecular systems

• DOI: 10.1242/jcs.02988
• Publication Date: 2006-05-24T03:34:58Z
• Authors: C. Sonnet; P. Lafuste; L. Arnold; M. Brigitte; F. Poron; F. Authier; F. Chrétien; R. K. Gherardi; B. Chazaud
• Keywords: Macrophage; Cell Survival; Muscle Fibers, Skeletal; Skeletal muscle; Apoptosis; In Vitro Techniques; Myoblasts; Mice; 03 medical and health sciences; [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]; Cell Adhesion; Animals; Humans; Muscle, Skeletal; Cells, Cultured; Elapid Venoms; Muscle Cells; 0303 health sciences; Cell adhesion molecules; Macrophages; Cell Membrane; Flow Cytometry; Mice, Inbred C57BL; Myogenic precursor cells; Cell Adhesion Molecules; Signal Transduction

The mechanisms underlying stromal cell supportive functions are incompletely understood but probably implicate a mixture of cytokines, matrix components and adhesion molecules. Skeletal muscle uses recruited macrophages to support post-injury regeneration. We others have previously shown that secrete mitogenic factors for myogenic cells. Here, we focused on macrophage-elicited survival signals. demonstrated that: (1) macrophage influx is temporally correlated with the disappearance TUNEL-positive apoptotic cells during regeneration in mice; (2) direct cell-cell contacts between human rescue from apoptosis, as assessed by decreased annexin V labelling caspase-3 activity, increased DIOC-6 staining, Bcl-2 expression phosphorylation Akt ERK1/2 pathways; (3) four pro-survival molecular systems detected DNA macroarray expressed vitro vivo - VCAM-1-VLA-4, ICAM-1-LFA-1, PECAM-1-PECAM-1 CX3CL1-CX3CR1; (4) deliver anti-apoptotic signals through all systems, functional analyses blocking antibodies; (5) more strongly differentiated myotubes, which must achieve adhesion-induced stabilisation their structure survive. Macrophages could secure these until they establish final association matrix.