Differential regulation of adherens junction dynamics during apical–basal polarization

• DOI: 10.1242/jcs.086694
• Publication Date: 2011-12-09T05:05:08Z
• Authors: Juan Huang; Lynn Huang; Yi-Jiun Chen; Erin Austin; Caitlin E. Devor; Fabrice Roegiers; Yang Hong
• Keywords: Adherens junction; Dynamics; Basal (medicine)

Adherens junctions (AJs) in epithelial cells are constantly turning over to modulate adhesion properties under various physiological and developmental contexts, but how such AJ dynamics regulated during the apical–basal polarization of primary epithelia remains unclear. Here, we used new genetically validated GFP markers Drosophila E-cadherin (DE-cadherin, hereafter referred as DE-Cad) β-catenin (Armadillo, Arm) quantitatively assay vivo biosynthetic turnover membrane redistribution by fluorescence recovery after photobleaching (FRAP) assays. Our data showed that DE-Cad Arm AJs polarizing had much faster than polarized cells. Fast is independent actin- dynamin-based trafficking, microtubule-dependent. Furthermore, a diffusion-based was both qualitatively different from slower exchange-based distribution, indicating association with more dynamic cells, only becomes stable Consistently, biochemical assays binding weaker revealed molecular interaction between modulated polarization, suggesting mechanism might be crucial for establishing polarity through regulating dynamics.