Fission yeast Kinesin-8 controls chromosome congression independently of oscillations

• DOI: 10.1242/jcs.160465
• Publication Date: 2015-09-11T05:16:06Z
• Authors: Hadrien Mary; Jonathan Fouchard; Guillaume Gay; Céline Reyes; Tiphaine Gauthier; Clémence Gruget; Jacques Pécréaux; Sylvie Tournier; Yannick Gachet
• Keywords: Kinesin

In higher eukaryotes, efficient chromosome congression relies, among other players, on the activity of chromokinesins. Here, we provide a quantitative analysis kinetochore oscillations and positioning in S. Pombe, model organism lacking wild type cells, chromosomes align during prophase while oscillating, maintain this alignment throughout metaphase. Chromosome are dispensable both for stable Kinesin-8 controls by regulating their oscillations. Oppositely, demonstrate that fission yeast an alternative mechanism. We propose aligns controlling pulling forces length dependent manner. A coarse grained segregation implemented with length-dependent process force at kinetochores is necessary sufficient to mimic prevents appearance lagging chromosomes. Altogether, these data illustrate how local action motor protein provides spatial cues within spindle prevent aneuploidy.