Direct neutrophil and T cell contact with macrophages induces release of phagosomally processed PAMPs via eructophagy

• DOI: 10.1242/jcs.263731
• Publication Date: 2025-02-28T14:21:06Z
• Authors: Jenny A. Nguyen; Tanis L. Orsetti; Philip Vernon; Catherine J. Greene; Neil McKenna; Robin M. Yates

Macrophages play a pivotal role in clearing debris and microbes from the microenvironment via phagocytosis orchestrating local inflammation. While mostly understood to be through synthesis secretion of soluble mediators such as cytokines eicosanoids, it has been recently proposed that macrophages can release previously phagocytosed processed PAMPs DAMPs into process termed eructophagy, these, turn, activate vicinal leukocytes. Additionally, commonly observed physically interact with other leukocytes, neutrophils T cells, recruited sites This study demonstrated eructophagy is significantly induced during physical interaction which mediated by ICAM1 on LFA1 neutrophils/T cells. Notably, activation alone sufficient trigger dependent Lyn kinase. Through this mechanism, lymphocytes influence their own interacting containing PAMP-containing phagolysosomes, subsequently triggers PAMP eructophagy.