Increase in intracellular pH induces phosphorylation of axonemal proteins for activation of flagellar motility in starfish sperm

• DOI: 10.1242/jeb.01906
• Publication Date: 2005-12-09T18:23:20Z
• Authors: Ayako Nakajima; Masaya Morita; Akihiro Takemura; Shinji Kamimura; Makoto Okuno
• Keywords: Intracellular pH; Axoneme

SUMMARY Increased intracellular pH ([pH]i) activates dynein in sea urchin and mammalian sperm induces activation of flagellar motility. It is thought that cAMP-dependent protein phosphorylation associated with motility through increasing [pH]i, but little attention has been given to the cAMP-independent also induced by the[pH]i increase. The present study demonstrates increase in[pH]i starfish axonemal proteins independently cAMP. Flagellar intact was activated when [pH]i raised addition NH4Cl. Histidine, which known activate sperm, during activation. In addition, demembranated flagella a pH-dependent manner without These results indicate it Moreover, (of molecular mass 25, 32 45 kDa) observed sperm. This suggests regulates via proteins.