In vivoandin vitroassessment of cardiac β-adrenergic receptors in larval zebrafish (Danio rerio)

• DOI: 10.1242/jeb.052803
• Publication Date: 2011-04-13T17:15:09Z
• Authors: Shelby L. Steele; Xiaodi Yang; Mélanie Debiais-T...; Thorsten Schwerte; Bernd Pelster; Marc Ekker; Mario Tiberi; Steve F. Perry
• Keywords: Danio; HEK 293 cells; Morpholino

SUMMARY β-Adrenergic receptors (βARs) are crucial for maintaining the rate and force of cardiac muscle contraction in vertebrates. Zebrafish (Danio rerio) have one β1AR gene two β2AR genes (β2aAR β2bAR). We examined roles these larval zebrafish vivo by assessing impact translational knockdown on function. larvae lacking expression morpholino displayed lower heart rates than control fish, whereas deficient both β2aAR β2bAR exhibited significantly higher controls. These results suggested a potential inhibitory role or genes. By using cultured HEK293 cells transfected with βARs, we demonstrated that stimulation adrenaline procaterol (a agonist) resulted an increase intracellular cAMP levels expressing any three βARs. In comparison its human βAR counterpart, expressed appeared to exhibit unique binding affinity profile adrenergic ligands. Specifically, had high phenylephrine, classical α-adrenergic receptor agonist. The also distinct ligand affinities agonists when compared βARs culture, being from β2bAR. Overall, this study provides insight into function evolution fish mammalian β-adrenergic receptors.