Molecular cloning and sequence ofSparus aurataskeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation

• DOI: 10.1242/jeb.204.17.3009
• Publication Date: 2021-04-23T23:44:10Z
• Authors: Katerina A. Moutou; Adelino V. M. Can...; Zissis Mamuris; Deborah M. Power
• Keywords: Thyroid Hormones; DNA, Complementary; Myosin Light Chains; Molecular Sequence Data; Development; Sea bream; 03 medical and health sciences; Sparus aurata; Animals; Amino Acid Sequence; Cloning, Molecular; Muscle, Skeletal; Phylogeny; Gene Library; 0303 health sciences; Base Sequence; Reverse Transcriptase Polymerase Chain Reaction; Gene Expression Regulation, Developmental; Sequence Analysis, DNA; Myosin light chain; Perciformes; 3. Good health; White muscle; Thyroxine; Thyroid regulation; Triiodothyronine; Sequence Alignment

SUMMARY Two full-length cDNA clones encoding the skeletal myosin light chain 2 (MLC2; 1452bp) and 3 (MLC3; 972bp) were isolated from a library prepared gilthead sea bream Sparus aurata larvae. The MLC2 encoded predicted protein of 170 residues that was 79% identical to rabbit over entire length 87% within Ca2+-binding region. deduced amino acid sequence MLC3 153 in 91% 69% zebrafish MLC3, respectively. Northern blot analysis revealed adults both transcripts expressed fast white muscle only. appeared earlier development: detectable beginning segmentation, whereas did not appear until 27h post-fertilisation. At this developmental stage, second transcript 0.89 kilobase-pairs present. MLCs exhibited different age-related pattern response varied thyroidal states, which experimentally induced by administration 1μgg−1bodymass thyroxine (T4) or triiodothyronine (T3), 5ngg−1bodymass hypothyroidal compound thiourea; expression significantly affected, levels elevated only juvenile after T4. Although mechanism regulation MLC remains unknown, present results suggest regulatory mechanisms exist for MLCs.