The osmorespiratory compromise in the euryhaline killifish: water regulation during hypoxia

• DOI: 10.1242/jeb.204818
• Publication Date: 2019-08-29T19:45:11Z
• Authors: Chris M. Wood; Ilan M. Ruhr; Kevin L. Schauer; Yadong Wang; Edward M. Mager; Danielle McDonald; Bruce Stanton; Martin Grosell
• Keywords: Gills; Male; Salinity; Acclimatization; drinking; Fresh Water; Fundulus heteroclitus; Osmoregulation; Fundulidae; PEG-4000; Animals; Seawater; Diffusive Water Flux; Anaerobiosis; 14. Life underwater; freshwater; seawater; Transcellular Permeability; gills; ventilation; Water; 04 agricultural and veterinary sciences; Tritiated Water; 6. Clean water; 3. Good health; Oxygen; 0401 agriculture, forestry, and fisheries; Female

Freshwater- and seawater-acclimated Fundulus heteroclitus were exposed to acute hypoxia (10% air saturation, 3h), followed by normoxic recovery (3h). In both salinities, ventilation increased and heart rate fell in the classic manner, while MO2 initially declined by ∼50%, with partial restoration by 3h of hypoxia, and no O2 debt repayment during recovery. Gill paracellular permeability (measured with [14C] PEG-4000) was 1.4-fold higher in seawater, and declined by 50% during hypoxia with post-exposure overshoot to 188%. A similar pattern with smaller changes occurred in freshwater. Drinking rate (also measured with [14C] PEG-4000) was 8-fold higher in seawater fish, but declined by ∼90% during hypoxia in both groups, with post-exposure overshoots to ∼270%. Gill diffusive water flux (measured with 3H2O) was 1.9-fold higher in freshwater fish, and exhibited a ∼35% decrease during hypoxia, which persisted throughout recovery, but was unchanged during hypoxia in seawater fish. Nevertheless, freshwater killifish gained weight while seawater fish lost weight during hypoxia and these changes were not corrected during normoxic recovery. We conclude that this hypoxia-tolerant teleost beneficially reduces gill water permeability in a salinity-dependent fashion during hypoxia, despite attempting to simultaneously improve MO2, but nevertheless incurs a net water balance penalty in both freshwater and seawater.