Removal of .BETA.III Isotype Enhances Taxol Induced Microtubule Assembly.

• DOI: 10.1247/csf.18.173
• Publication Date: 2009-10-23T04:38:43Z
• Authors: Qing Lu; Richard F. Luduena
• Keywords: Isotype; BETA (programming language)

The interaction of beta III-depleted tubulin with taxol was investigated. A monoclonal antibody against the III isotype immobilized on a sepharose 4B column and used to remove from unfractionated tubulin. assembly in presence enhanced compared critical concentration 10 microM is 0.4 mg/ml, while 0.16 mg/ml. At different taxol, increased relative that reaches maximum at about 1:1 ratio taxol. has also been studied by electron microscopy. After 2 minutes 37 degrees C, results only ribbon-like ring structures; there are no visible microtubules. By 5 minutes, microtubules appear increase length. occurs more quickly. In contrast case tubulin, assembles within into which length time. 30 assembled shorter than There difference between Taxol-induced resistant inhibiting effect podophyllotoxin colchicine. It less sensitive cold temperature.