Differential Gene Expression in High and Low active Animals.

• DOI: 10.1249/01.mss.0000389463.93642.73
• Publication Date: 2010-09-24T17:16:17Z
• Authors: J. Timothy Lightfoot; Alicia Hamilton; Trudy Moore-Harrison
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences

It is well established that physical activity levels are significantly controlled by genetic factors; however, the specific genes and mechanisms involved in regulation of unknown. PURPOSE: To compare gene expression pattern several potential candidate both nucleus accumbens (brain) gastrocnemius/soleus (G/S muscle) between two differentially active inbred strains mice. METHODS: From previous experiments, acumbens G/S tissue were used from 11 high C57L/J (LJ) mice (8 male, 3 female) 13 low C3H/HeJ (C3H) 5 female). As we had seen previously, while there was no difference weight (LJ=23.7±2.6 gms, avg±SD vs. C3H=23.3±3.6 gms), over 21 days wheel running activity, LJ ran farther (11.1±3.0 km/day 1.6±2.1 km/day), longer (367.2±56.3 min/day 92.3±112.7 min/day), faster (30.3±5.9 m/min 14.6±2.9 m/min) than C3H proving higher daily Standard quantitative RT-PCR techniques developed our lab to determine differential previously suggested genes: dopamine receptor 2 (Drd2), nescient helix-loop-helix (Nhlh2), alpha actinin (Actn2), (Actn3), melanocortin-4-receptor (Mc4r), aromatase (Cyp19a1), leptin (Lepr), myostatin (Mstn), 3'-phosphoadenosine 5'-phosphosulfate synthase (Papss2), glucose transporter 4 (Slc2a4). All data log transformed for normality purposes values compared using one-way ANOVAs within each with value set = 0.05. RESULTS: Of comparisons brain tissue, Lepr (p=0.02) Mstn (p=0.0001) expressed at In Nhlh2 (p=0.05) Slc2a4 (p=0.0005) CONCLUSIONS: Our suggest animals. While this does not establish a role any these regulation, it show an association activity; further research should delineate. Supported NIH R01AR050085 Huffines' Institute.