Effect of stem cells seeded onto biomaterial on the progression of experimental chronic kidney disease

• DOI: 10.1258/ebm.2011.011024
• Publication Date: 2011-05-24T02:54:21Z
• Authors: Heloisa C Caldas; Ida M M Fernandes; Rosa S Kawasaki-O...; Maria Alice S F B...; Ana Maria G Plepis; Virginia A Martins; Terezila M Coimbra; Eny M Goloni-Bert...; Domingo M Braile; Mario Abbud-Filho
• Keywords: Glomerulosclerosis; Cell therapy

Different routes for the administration of bone marrow-derived cells (BMDC) have been proposed to treat progression chronic renal failure (CRF). We investigated whether (1) use bovine pericardium (BP) as a scaffold cell therapy would retard CRF and (2) efficacy differently impacts distinct degrees CRF. used 2/3 5/6 models mass reduction simulate different stages chronicity. Treatments consisted BP seeded with either mesenchymal or mononuclear implanted in parenchyma remnant kidney. Renal function proteinuria were measured at days 45 90 after implantation. BMDC treatment reduced glomerulosclerosis, interstitial fibrosis lymphocytic infiltration. Immunohistochemistry showed decreased macrophage accumulation, proliferative activity expression fibronectin α-smooth muscle-actin. Our results demonstrate: biomaterial combined did experimental CRF; cellular stabilized serum creatinine (sCr), improved clearance 1/sCr slope when administered during less severe (3) fibrogenic molecules; (4) biomaterials can be an alternative route therapy.