RNase III-independent microRNA biogenesis in mammalian cells

• DOI: 10.1261/rna.036194.112
• Publication Date: 2012-10-25T03:32:40Z
• Authors: Thomas Maurin; Demián Cazalla; Jr-Shiuan Yang; Diane Bortolamiol...; Eric C. Lai
• Keywords: Drosha; Dicer; Argonaute; Ribonuclease III

RNase III enzymes are fundamental to the biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs) in all species studied. Although alternative miRNA pathways independent Drosha or Dicer exist, each still requires one III-type enzyme. Here, we describe two strategies that marry either Z Integrator complex with slicing activity Argonaute2 generate highly functional mature miRNAs. We provide stringent validation their independence by demonstrating efficient knockout cells. These data proof-of-principle evidence for additional mechanistic possibilities generation regulatory RNAs, represent novel silencing triggers may be exploited technical purposes.