Identification of Key Genes and Pathways Associated with RUNX1 Mutations in Acute Myeloid Leukemia Using Bioinformatics Analysis

• DOI: 10.12659/msm.910916
• Publication Date: 2018-10-05T17:14:39Z
• Authors: Fangxiao Zhu; Rui Huang; Jing Li; Xiwen Liao; Yumei Huang; Yongrong Lai
• Keywords: KEGG; RUNX1

BACKGROUND RUNXl plays a key regulatory role in the process of hematopoiesis and is common target for multiple chromosomal translocations human acute leukemia. Mutations RUNX1 gene can lead to leukemia affect prognosis AML patients. We aimed identify pivotal genes pathways involved RUNX1-mutated patients with myeloid (AML) explore possible molecular markers novel therapeutic targets disease. MATERIAL AND METHODS The RNA sequencing datasets 151 cases were obtained from Cancer Genome Atlas database. Differentially expressed (DEGs) identified using edgeR R platform. PPI (protein-protein interaction) network clustering modules analyzed ClusterONE, KEGG (Kyoto Encyclopedia Genes Genomes) pathway enrichment analyses performed. RESULTS A total 379 as DEGs. analysis DEGs showed significantly enriched cancer, extracellular matrix (ECM)-receptor interaction pathway, cyclic adenosine monophosphate (cAMP) signaling pathway. top 10 ranked by degree PRKACG, ANKRD7, RNFL7, ROPN11, TEX14, PRMT8, OTOA, CFAP99, NRXN1, DMRT1, which hub protein-protein (PPI). Statistical revealed that had shorter median survival time (MST) poor clinical outcome an increased risk death when compared those without mutations. CONCLUSIONS present study will help understand mechanisms underlying mutations develop effective strategies RUNX1-mutation AML.