Multiple Cytokine Analysis of Th1/Th2/Th9/Th17/Th22/Treg Cytokine Pathway for Individual Immune Profile Assessment in Patients with Psoriasis

• DOI: 10.12659/msm.938277
• Publication Date: 2022-11-07T14:15:08Z
• Authors: Anna Michalak-Stoma; Joanna Bartosińska; Dorota Raczkiewicz; Małgorzata Kowal; Joanna Kozak; Mariusz Gujski; Dorota Krasowska; Grażyna Chodorowska
• Keywords: Interleukin-23; Interleukin 9

BACKGROUND:Psoriasis is an autoimmune and autoinflammatory disorder that has a significant impact on patient quality of life. The aim the study was to assess immune profiles patients with psoriasis multiple cytokine analysis. MATERIAL AND METHODS:Fifty-two male psoriatic 24 healthy volunteers were recruited. Granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon gamma (IFN-gamma), interleukin (IL)-1 beta, IL-2, Il-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, tumor necrosis (TNF)-alpha measured in patients’ serum Th1/Th2/Th9/Th17/Th22/Treg Cytokine 18-Plex Human ProcartaPlex Panel, based Luminex xMAP technology. RESULTS:The median fluorescence intensities GM-CSF, IL-22 not intensive enough calculate concentration. We observed elevated levels IL-6 (P=0.001) IL-9 (P=0.003) patients, compared control group. IL-1beta (P=0.008) IL-27 (P=0.006) decreased. In arthritis, we noticed decreased level without arthritis (P=0.034). IL-12 (P<0.05) IL-18 correlated positively Psoriasis Area Severity Index. found negative correlations (P<0.05), IL-23 age patients; duration; Nail CONCLUSIONS:Multiple analysis seems be important form individual profile assessment before treatment selection.