A MODIFIED METHOD OF THE PRUSSIAN BLUE REACTION FOR THE HISTOCHEMICAL DEMONSTRATION OF IRON, AND ITS APPLICATION TO THE COLLOIDAL IRON REACTION OF ACID MUCOPOLYSACCHARIDES

• DOI: 10.1267/ahc.3.18
• Publication Date: 2011-08-19T02:46:50Z
• Authors: SHUNTA HIROSE; MASAYUKI YASUTOMI; NOBUHIRO MURAI; RYOTARO YAMADA; ATSUSHI KATAYAMA; MOTOYOSHI TSUJINO; ZENJI IWASA; A.K.A. RAZZAQ
• Keywords: 0301 basic medicine; 03 medical and health sciences

In the Prussian Blue method for the histochemical demonstration of iron, some parts of produced pigment are considerably washed away during the dyeing process, because the pigment is water soluble. Consequently decrease and diffusion of the pigment occur and staining of the surrounding tissues take place. The color reaction was carried out in 70% acetone solution in order to decrease the solubility of the pigment. Obtaining the sharp and clear demarcation, this method was applied successfully to the colloidal iron reaction of acid mucopolysaccharides and very strong and clear reaction was obtained.