A MODIFIED METHOD OF THE PRUSSIAN BLUE REACTION FOR THE HISTOCHEMICAL DEMONSTRATION OF IRON, AND ITS APPLICATION TO THE COLLOIDAL IRON REACTION OF ACID MUCOPOLYSACCHARIDES

• DOI: 10.1267/ahc.3.18
• Publication Date: 2011-08-19T02:46:50Z
• Authors: SHUNTA HIROSE; MASAYUKI YASUTOMI; NOBUHIRO MURAI; RYOTARO YAMADA; ATSUSHI KATAYAMA; MOTOYOSHI TSUJINO; ZENJI IWASA; A.K.A. RAZZAQ
• Keywords: Prussian blue

In the Prussian Blue method for histochemical demonstration of iron, some parts produced pigment are considerably washed away during dyeing process, because is water soluble. Consequently decrease and diffusion occur staining surrounding tissues take place. The color reaction was carried out in 70% acetone solution order to solubility pigment. Obtaining sharp clear demarcation, this applied successfully colloidal iron acid mucopolysaccharides very strong obtained.