The identification of high-performing antibodies for Sequestosome-1 for use in Western blot, immunoprecipitation and immunofluorescence

• DOI: 10.12688/f1000research.132628.2
• Publication Date: 2024-07-11T14:36:59Z
• Authors: Riham Ayoubi; Walaa Alshafie; Irina Shlaifer; Kathleen Southern; Peter S. McPherson; Carl Laflamme
• Keywords: Sequestosome 1; Immunofluorescence; Immunoprecipitation

<ns3:p>Sequestosome-1, encoded by the gene <ns3:italic>SQSTM1</ns3:italic>, functions as a bridge between ubiquitinated proteins and proteasome or autophagosome, thereby regulating protein degradation pathways. Loss of Sequestosome-1 is hypothesized to enhance neurodegeneration progression in several diseases, including amyotrophic lateral sclerosis (ALS) frontotemporal disorders (FTD). reproducible research would be facilitated with availability well-characterized anti-Sequestosome-1 antibodies. In this study, we characterized seventeen commercial antibodies for Western blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. We identified many high-performing encourage readers use report guide select most appropriate antibody their specific needs.</ns3:p>